Genetically encoded photoaffinity histone marks

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发表刊物:JACS

摘要:Posttranslational modifications (PTMs) of lysine are crucial histone marks that regulate diverse biological processes. The functional roles and regulation mechanism of many newly identified lysine PTMs, however, remain yet to be understood. Here we report a photoaffinity crotonyl lysine (Kcr) analogue that can be genetically and site-specifically incorporated into histone proteins. This, in conjunction with the genetically encoded photo-lysine as a “control probe”, enables the capture and identification of enzymatic machinery and/or effector proteins for histone lysine crotonylation.

合写作者:Xiao Xie,# Xiao-Meng Li,# Fangfei Qin,# Jianwei Lin, Gong Zhang, Jingyi Zhao, Xiucong Bao, Rongfeng Zhu, Haiping Song, Xiang David Li,* and Peng R. Chen*

第一作者:X. Xie,# X. Li,# F. Qin,# X. Li*, and P. Chen

通讯作者:Xiang David Li,* and Peng R. Chen*

卷号:139

期号:19

页面范围:6522-6525

是否译文:否

CN号:null

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